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Fig. 2. Perturbation of any single cytoskeletal system in unfixed samples does not prevent interaction of the remaining two. Triple label confocal micrographs showing that inhibition of F-actin (green) fails to prevent cytokeratin (blue) association with aster microtubules (red) (latrunculin, arrows); inhibition of cytokeratin polymerization does not inhibit F-actin association with aster microtubules (C11, arrows); inhibition of microtubule polymerization does not prevent interactions between F-actin and cytokeratin (nocodazole, arrows).