Fig. 6. Demonstration of functional nuclear localization signal within the N-terminus of p120ctn. Both expression constructs, N1 and N3, localized in the nucleus (A-D), whereas the C-terminally deleted N1A (see Fig. 4) demonstrated diffuse staining throughout the cell (E,F). Transiently transfected 1205-Lu cells were detected with MAb M2 (A,C,E) and the nuclei demonstrated with DAPI (B,D,F). Bar, 50 µm.