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Fig. 2. Phenotypes of pph-4.1(RNAi) embryos at the one-cell stage. All embryos are shown with the anterior end to the left. (A) A wild-type one-cell embryo showing a male pronucleus (black arrowhead) and a female pronucleus. (B) A wild-type embryo with a bipolar spindle. (C) A pph-4.1(RNAi) embryo with two male pronuclei (black arrowheads). (D) A pph-4.1(RNAi) embryo showing a tetra-polar spindle. (E) A pph-4.1(m-/p+) embryo with a tetra-polar spindle. Three spindle poles are visible in this focal plane. Spindle poles in B, D and E are indicated by white arrowheads. Bar, 10 µm.