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Fig. 6. Loss of PPH-4.1 function affects the localization of PLK-1 during mitosis. (A-C) Wild-type one-cell embryo at prometaphase. (D-F) pph-4.1(RNAi) one-cell embryo after pronuclear meeting. (A,D) DAPI staining showing chromosomes. (B,E) Anti-{alpha}-tubulin antibody staining. (C,F) Anti-PLK-1 antibody staining. White arrowheads indicate centrosomal staining of PLK-1. Black arrowheads indicate perinuclear PLK-1 staining. Black arrows indicate cytoplasmic granules. In pph-4.1(RNAi) embryos, PLK-1 did not localize to centrosomes even though chromosomes are condensed. Embryos are aligned with the anterior end to the left. Bar, 10 µm.