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Fig. 7. Decreased levels of FEH-1 or APL-1 protein result in increased pharyngeal pumping. (A) The rate of pharyngeal contractions were determined in (+/+) individuals (N2), in feh-1 (+/gb561) heterozygous worms (+/-), as well as in feh-1(gb561/gb561) individuals rescued by feh-1 transgene (R). Significance of the difference between wild-type and feh-1 (+/gb561) individuals was evaluated using the Student's t test (P<0.0001). Bars indicate standard deviation. (B) The rate of pharyngeal contractions was determined in wild-type Bristol N2 individuals fed to bacteria producing vector-derived ds RNA (vec) or vector/feh-1 dsRNA (feh) or vector/apl-1 dsRNA (apl). Significance of results obtained from feh-1 and apl-1 dsRNAs versus control measurementes was evaluated using the Student's t test (P<0.0001). Bars indicate standard deviation.