Fig. 4. WT NR6 cell adhesion strength on fibronectin-coated for BSA-coated substrates was measured after a 12 hour incubation in serum-free medium followed by an 8 hour incubation in medium containing 1% dialyzed serum. Adhesion dependence on ligand density and ligand specificity is greatly diminished, and cell detachment is insensitive to the range of detachment forces applied.