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Fig. 1. The two-step approach to generate cells expressing IC-GFP in an IC-null background. (a) Step 1, introduction of an IC-GFP expression construct into wild-type background. (b) Step 2, targeting the endogenous dic gene by homologous recombination in cells stably expressing IC-GFP using the Thy1 selectable marker. (c) The dic gene is replaced by thy1 and the only IC expressed is the IC-GFP fusion.