Fig. 3. Dynein-GFP colocalizes with microtubules. Immunofluorescence staining of IC-GFP-expressing cells with an anti-tubulin antibody followed by Lissamine-conjugated secondary antibody. (a) Dynein-GFP signal. (b) Tubulin staining. (c) Overlay of (a) and (b), with GFP in green and tubulin staining in red. Yellow indicates colocalization. The arrowhead points to an example of punctate dynein-GFP signals lining along a microtubule. The arrow points to an example of dynein-GFP signal associated with microtubule plus end.