Fig. 3. Analysis of the number of satellite cells emanating from single fibers in the presence of serum. (A) Data are expressed as the cumulative rank of the data from single fibers grown in DMEM containing 0.1% HS and either non-irradiated (left panel) or LELI (right panel) (n=200). Curves were plotted as described in Fig. 2. The population distribution of LELI cells is significantly different from control cells on all days (Kruskal-Wallis ANOVA median test; Rao R (4,99)=9.65; P<0.001). (B) A synergistic effect of LELI and serum on cell accumulation around the fibers. Data are presented as the percentage change of the total cell number that was pooled from all experiments in each treatment relative to day 1. White bars, non-irradiated fibers were grown in 0.1% HS-DMEM. Black bars, fibers were irradiated and kept in serum-free DMEM. Hatched bars, fibers were subjected to LELI and kept in DMEM-0.1% HS.