Fig. 4. Effect of LELI on anti- and pro-apoptotic proteins. Two fibers were plated on each Petri dish: one was irradiated and the other served as a control. One day post irradiation, fibers were fixed and stained for BAX (A, C) or Bcl-2 (B, D) and counter-stained with DAPI. Bar, 30 µM. (E) Western blot analysis of Bcl-2, BAX, p53 and p21 in control and irradiated i28 myogenic cells on various days post-irradiation. Equal quantities of proteins were loaded as evidenced by staining for -tubulin. (F) Densitometric analysis of protein expression levels normalized to that of -tubulin. Results are averages of two independent repeats.