Fig. 2. Adhesion of FL-DG- or ßDG-expressing BAE to dishes coated with laminin-1 or fibronectin. Cells were transfected and selected for resistance against geneticin. The adhesion assay was performed as described in the Materials and Methods in the presence or absence of 2 mg/ml heparin. During the early phase of cell attachment (2 hours), the extent of cell spreading on the substrate was similar in all cases. Each bar represents the means±s.e.m. of three determinations, each done in triplicate. The values are expressed relative to the estimated number of the attached vector-transfected cells in the absence of heparin (100%). ^* p<0.01; significantly different from the values of vector-transfected cells.