Fig. 4. Proliferation of FL-DG- or ßDG-expressing BAE. (A) Photomicrographs of confluent monolayers of cells transfected with vector alone (vector), a full-length DG construct (FL-DG) or a construct of the cytoplasmic tail of DG (ßDG). Cells were photographed after 7 days in culture. (B) Quantification of the cell surface area. The cell surface area was measured from images similar to the ones shown in (A) using an NIH image software. Each bar represents the mean ±s.e.m. of the values from more than 100 cells obtained in three independent experiments. (C) Proliferation curves. Transfected cells were seeded in 24-well plates at a low density and allowed to proliferate in DMEM/10% FCS. The mean numbers of cells of triplicate determinations obtained in a single experiment are shown. Similar results were obtained on two other occasions.