Fig. 6. Changes in expression levels of ßDG in synchronized cultures of BAE as the cells progress through a cell cycle. Cells were arrested at G₀/G₁ by serum-deprivation and then stimulated with 5% FCS to allow cell cycle progression for up to 24 hours. (A) Cell cycle analysis by flow cytometry. (B) Anti-ßDG western blotting of membrane preparations. Anti-actin immunoblot is used to as loading control. Both in (A) and (B), the results obtained in a single experiment are shown, but similar findings were obtained in two other independent experiments.