Fig. 7. Anti-ßDG immunohistochemistry of surgical human specimens. Bound antibodies were visualized with HRP-conjugated secondary antibody and DAB (brown), and sections were also counterstained with hematoxylin. (A-F) Kidney cancer. Shown are the photomicrographs of cancer tissues (B,D,F) and of normal tissue (A,C,E) that surrounds the tumor. The venules boxed in (A) and (B) are enlarged in (C) and (D), respectively. Arrows point to endothelial cells. The slide from which (E) and (F) were taken was treated with DAB for a longer period of time (see non-specific background staining of red blood cells), but VEC of the vessels found in the normal tissue were not labeled (E). (G,H) Colon cancer. Photomicrographs of arteriole s found in the cancer (H) and the normal tissue (G) that surrounds the tumor are shown.