Fig. 2. Analysis of GFP8 cells with antibodies for acetylated tubulin. Top: isolated cytoskeleton of a control cell stained with polyclonal anti--tubulin and 6-11B-1 (acetylated tubulin), revealing the numerous microtubules of the cytoskeleton and the four acetylated flagellar roots. Bottom: GFP8 cytoskeletons stained with 6-11B-1 and corresponding GFP images, revealing that prominent SFA-GFP fibers were mostly associated with microtubular bundles characterized by short acetylated regions. The arrowheads mark microtubular roots acetylated over most of their length. Bar, 10 µm.