Fig. 11. Prob and Mt interact in Sf9 cells. Cells were co-infected with recombinant baculovirus containing Prob and Mt. At 72 hours post-infection, cells were lysed. Total cell lysates were immunoprecipitated with either the anti-MAP2 antibody, or with AP20 or HM2, which recognize Prob. The presence of Mt in the immunoprecipitates was analyzed by immunoblotting using the antibody 46.1. In lane 1, no antibody was added to the cell lysates prepared from Prob/Mt-expressing cells. In lane 2, the membrane was stained with AP20 to reveal the presence of Prob in the immunoprecipitates. In lane 3, the membrane probed with AP20 was stripped and stained with the antibody 46.1 to reveal the presence of Mt in the immunoprecipitates (arrowhead). In lane 4, Mt was immunoprecipitated using the antibody 46.1 from cells expressing only Mt. In lane 5, no antibody was added to the cell lysates prepared from Prob-expressing cells. In lane 6, the membrane was stained with AP20 to reveal the presence of Prob in the immunoprecipitates. In lane 7, the membrane probed with AP20 was stripped and stained with the antibody 46.1 to reveal the presence of Mt in the immunoprecipitates. No band corresponding to Mt was found in the immunoprecipitates. The arrow indicates the immunoglobulins.