(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 6. Colocalization of CAP1 with cofilin, and analysis of factors that determine its localization.

(A,B) Spreading mouse C3H-2K fibroblasts were fixed and incubated with a rat anti-CAP1 IgG and a rabbit antibody to actin (A) or cofilin (B), followed by labelling with a Cy3-labeled anti-rat IgG and a fluorescein-labeled anti-rabbit IgG. The lamellipodia are indicated by arrowheads, whereas the ruffling areas are indicated by arrows.

(C) HA-tagged CAP1-NT was transiently expressed in C3H-2K cells (panels a-d) by lipofection. HA-tagged CAP1-CT (panels e and f) or HA-tagged CAP2-NT (panels g and h) were expressed as well. Transfected cells were split onto coverslips, and the spreading cells were fixed and reacted with a mouse anti-HA and a rabbit antibody to actin (panels a, b, e and f) or to cofilin (panels c, d, g and h), after which they were stained with a Cy3-labeled anti-mouse IgG and a fluorescein-labeled anti rabbit IgG. The lamellipodia are indicated by arrowheads (for CAP-NTs) or arrows (for CAP1-CT). The white bar represents 10 µm.