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Fig. 2. Mad2 on unattached kinetochores. Mad2 was localized in the nda3-KM311 mutant cells that were expressing Mis6-HA (top panels). Mis6-HA was visualized by indirect immunofluorescent staining (red), DNA by DAPI (blue) and Mad2 by GFP (green). As a negative control, indirect immunofluorescent staining for HA epitope was performed with cells not expressing Mis6-HA (lower panels).