Fig. 5. (A) The Slp1-Mad2 complex formed in nda3-KM311 mutant cells. The protein binding was observed by a coimmunoprecipitation experiment using the anti-Slp1 antibody in cdc25-22, nda3-KM311, nuc2, cut9 or mts3 mutants (left). Mad2-GFP and Slp1 were blotted using an anti-GFP (lower panel) or anti-Slp1 antibody (upper panel), respectively. The total amount of protein in the cells was analyzed by straight western blotting (right). (B) Visualization of Mad2 and Slp1 in nda3-KM311, cdc25-22, nuc2, cut9 or mts3 mutants. The cells were stained using an anti-Slp1 antibody (red) or DAPI (blue).

Mad2-GFP was observed in the same cells as those used for Slp1 staining and DAPI (green).