Fig. 1. Cadherin misexpression and cardiac hypertrophy in transgenic mice. (A) The MHC promoter was used to express either chicken N-cadherin or human E-cadherin specifically in the myocardium. Western blot analysis was performed on heart lysates from Ncad and Ecad transgenic mice using species-specific antibodies. GAPDH signal shows loading of samples between lanes. (B) Wholemount images of hearts removed from 4-week-old nontransgenic (left) and transgenic (right) littermates. Note the increased size of the MHC/Ecad heart in comparison to MHC/Ncad. (C) Bar graph illustrating the heart weight:body weight ratios of four transgenic lines compared with wild-type. C, chick heart; H, human keratinocyte; NT, nontransgenic. Bars, 2.5 mm (B).