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Fig. 4. Assessment of the `binucleated' phenotype in {alpha}MHC/Ecad transgenic mice. DAPI staining of sections from 4-week-old wild-type (A) and transgenic (B,C) hearts indicated an increase in `binucleated' hypertrophic nuclei (arrowheads) in the {alpha}MHC/Ecad mice. Many normal size nuclei were found in wild-type heart (A). In comparison, far fewer nuclei were observed in similar areas of hypertrophic myocardium in {alpha}Ncad4 (B) and {alpha}Ecad33 (C). The percentage of `binucleated' myocytes was assessed by counting several different fields containing a total of 150 hypertrophic myocytes (D). The `binucleated' phenotype was observed in other {alpha}MHC/Ecad transgenic lines such as E10. Indirect immunofluorescence of heart sections from 2-week-old animals indicated an increase in cyclin D1 (arrowheads) in {alpha}MHC/Ecad (F), but not {alpha}MHC/Ncad (E), consistent with the `binucleated' phenotype. Bar, 25 µm (A,B,C); 50 µm (E,F).