Fig. 6. Actin relocalisation in the mutants strains. 3 µg/ml of P factor was added at time 0. (A) Samples were taken every 30 minutes, fixed in formaldehyde for 40 minutes and stained for actin with rhodamine phalloidin. Actin localisation was scored for each time point. (B) Samples were taken every 2 hours, fixed in ethanol and processed for FACS analysis. (C) h90 wild-type and mutant strains were grown overnight in full nitrogen and low glucose medium (10 g/l) to 2x10⁷ cells/ml. Cells were then spun and re-suspended in low glucose, nitrogen-free medium at 1x10⁶ cells/ml to induce mating and fused cells were scored every hour. Experiments were repeated at least three times and at least 200 cells were scored for each time point. Standard deviation was calculated and error bars are shown.