Fig. 5. Cleavage embryos expressing lamin-GFP were injected at the posterior end (arrows) with wild-type (A-C) or P446L mutant importin-ß (D-F). A and D show localization of lamin-GFP during interphase following injection. The lamin-GFP molecules highlight the NE. Embryos are in metaphase in B and E, and the spindle envelopes are not affected (B,E). During the upcoming interphase, nuclear lamina re-forms in the embryos that were injected with normal importin-ß (C). No nuclear lamina assembles at the site of injection of the P446L mutant importin-ß revealing the failure of intact NE formation (F). Bar, 50 µm.