Fig. 1. Epifluorescence microscopy of cell lines stably expressing fluorescent human Dsc2a chimeras. Clone PDc-13 (A-C') was obtained after transfection of human hepatocellular carcinoma-derived PLC cells with construct C-Dsc2a.GFP-1 coding for fusion protein Dsc2a.GFP and selection with neomycin. Clone MDc-2 (D-H') was generated from canine kidney-derived MDCK cells by transfection with construct C-Dsc2a. YFP-2 coding for chimera Dsc2a. YFP and selection with hygromycin. The fluorescence elicited by the transgenic fusion proteins in methanol/acetone-fixed cells is shown in the micrographs on the left (A-H) and compared with the indirect immunofluorescence obtained after reaction with primary antibodies against GFP (anti-GFP), desmoplakin (anti-Dp), desmoglein (anti-Dsg), plakoglobin (anti-Pg), plakophilin 2 (anti-Pp2) and plakophilin 3 (anti-Pp3). Note the similar punctate fluorescence pattern in each picture pair, except for plakoglobin, which is detected in additional plasma membrane domains. Bars, 10 µm.