Fig. 10. Time-lapse epifluorescence microscopy of MDc-2 cells monitoring the distribution of Dsc2a. YFP in response to a transient 5 minute reduction of Ca²⁺. The entire sequence is shown as Movie 13 (jcs.biologists.org/supplemental ; 2 minute recording intervals). Note the uptake of fluorescent puncta into the cytoplasm shortly after the pulse of LCM and their continued presence in this location (small arrows), whereas small dots re-appear at the cell surface (large arrows). Bar, 10 µm.