Fig. 11. FRAP analyses in MDc-2 and PDc-13 cells. A representative series of photomicrographs of single confocal sections that were recorded with a large pinhole is depicted for MDc-2 (A) and PDc-13 cells (B). The bleached areas are demarcated by boxes. Note the strong desmosomal fluorescence before bleaching (prebleach); this fluorescence is completely destroyed by bleaching (bleached) but recovers to a considerable degree within 30 minutes (5 min, 30 min). Bars, 5 µm. (C) The graphs show digital representations of pooled results from MDc-2 (n=6) and PDc-13 cells (n=10). The means of the relative fluorescence intensities are blotted as functions of time. (D) Confocal fluorescence recording of bleaching in PDc-13 cells; microscope settings were adjusted for high spatial resolution (small pinhole). E,E' show a high magnification of the bleached area prior to bleaching and after a 30 minute recovery period. Note the re-emergence of fluorescence in desmosomal structures (arrows in D,E,E'). Bars, 10 µm in D, 1 µm in E'.