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Fig. 6. Epifluorescence microscopy of live MDc-2 monolayer monitoring the distribution of fluorescent chimera Dsc2a.YFP for 23.3 hours. The pictures are taken from a time-lapse recording (Movie 6; jcs.biologists.org/supplemental ), for which seven focal planes were imaged every 5 minutes. The projection images are shown. Individual cells are labeled by letters, and daughter cells that are generated during the observation period are denoted by ' and ''. Note the maintenance of desmosomes for very long periods (arrows). Bar, 10 µm.