Fig. 8. Double epifluorescence microscopy of live MDc-2 cells showing the distribution of Dsc2a.YFP and HK18-CFP during mitosis. Images at each time point represent the projection of six pictures (z-distance of 0.5 µm) recording the fluorescence emitted after excitation at 498 nm (YFP chimera) and 436 nm (CFP chimera). The complete series of images is provided as Movie 8 (jcs.biologists.org/supplemental ) (recording intervals 5 minutes; same color code as in Movie 5). Note the continued presence of Dsc2a. YFP-positive desmosomes throughout cell division, and the considerable alterations in the HK18-CFP-containing CKF system with some residual desmosome-associated material. Bar 5 µm.