JCS -- Gerald et al. 115 (8): 1749 Figure IG1

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Fig. 1. G1 cells respond to ${gamma}$ radiation. Wild-type cells were ${alpha}$ f arrested and irradiated or mock-treated then incubated at 22°C for 15 minutes before equilibrating on ice for processing. (A) Isolated chromosomes from ${alpha}$ f-arrested cells were probed with rabbit ${alpha}$ -Rfa1 (1:2000) then costained with DAPI and Alexa-488-conjugated ${alpha}$ -rabbit secondary (1:400). The superimposition of DAPI and Alexa fluorescence is shown. (B) 50 µg protein from ${alpha}$ f-arrested cells was run on 10% SDS-PAGE for western analysis. Blots were probed with ${alpha}$ -Rfa1 (1:1000) and visualized as described in the Materials and Methods. (C) 50 µg protein from ${alpha}$ f-arrested cells carrying RAD53-13Myc was run on 8% SDS-PAGE for western analysis.