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Fig. 10. Unbudded cells remain sensitive to {alpha}f. Wild-type cells (see Fig. 9A,B) were collected 18 hours after {gamma} (A) or UV (B) radiation and incubated in {alpha}f for 3.5 hours at 30°C. Cells were fixed as for flow cytometry and scored (X63, Zeiss Axioskop) for formation of mating projections (arrow). Mating projections were not observed after incubation without {alpha}f.