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Fig. 2. G1 {gamma} radiation results in dose-dependent replication delay. {alpha}f-arrested wild-type (A), rad9{Delta} and rad17{Delta} (B) cells were irradiated and released to media at 30°C. 0.5 ml aliquots were fixed at indicated time points for flow cytometry. Lines drawn across graphs show 1N and 2N positions. Identical results were obtained with {rho}0 cells (data not shown).