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Fig. 9. Unbudded G1 arrest can last for 18 hours after DNA damage. Log-phase strains were irradiated with 3000 Gy then diluted to ~104 cells/ml before plating on YPD (A) or were plated before UV irradiation at 100 J/m2 (B). Cell numbers were counted immediately (0 Hr) and at 18 hours post irradiation. (C) Cells were plated and incubated 0 or 20 minutes before 100 J/m2 irradiation. (Error bars represent s.d. of three experiments.)