Fig. 6. Cell size distribution of synchronized cells and multiple fission of asynchronous cells. (A) Wild type and fa2-2 mutant cells were grown in minimal media for several days in constant light, then placed in the dark for 24 hours. Cell sizes were measured immediately following exposure to light and again after 10 hours of continuous light. One hundred cells from each culture were measured. (B) Wild-type and fa2-2 mutant cells were grown in rich (TAP) media in constant light for several days. Aliquots of the culture were plated on minimal media and placed in the dark for 24 hours. Individual cells/colonies were scored for having undergone 0, 1, 2, 3 or 4 divisions during the 24 hour incubation in the dark by counting cells in micro-colonies. 300 cells were scored for each culture from each of two independent experiments.