Fig. 8. RNAi of FA2 in wild-type Chlamydomonas cells. (A) Construct for triggering RNAi. The construct pBSFA2-RNAi was created by cloning a cDNA cassette containing exons 1 to 5 in inverse orientation at the 3' end of the corresponding genomic DNA. (B) Northern analysis of FA2 in cells transformed with the pBSFA2-RNAi construct. Each lane contains 10 µg of polyadenylated RNA obtained from a population of asynchronous cells from the strain indicated. The blot was probed with 0.7 kb of FA2 cDNA and re-probed with 0.5kb of CBL as a loading control. (C) Ratio of FA2 mRNA signal compared with that of CBL. (D) Deflagellation response of each cell type to acid. (E) Cell size distribution of RNAi colony number 17. One hundred cells were measured.