Fig. 2. Western blot analysis of subfractions of the detergent insoluble fraction (DIF) of NIH 3T3 cells. Proteins from cell equivalents of cell lysate (CL), total DIF (DIF), nuclear matrix (NM) and actin-enriched fraction (AF) were separated on 10% SDS-PAGE and subsequently western blotted and incubated with antibodies directed against caveolin-1 (A), GM130 (B), actin (C) and lamin A/C (D).