Fig. 3. Lipid kinase activity in actin filaments and lamina-pore complexes. Proteins from actin filaments and lamina-pore complexes (both 50 µg) from NIH 3T3 cells were used in a lipid kinase assay as described in the legend to Fig. 1, and the PtdIns(4)P spots were quantified (actin filaments: s.e.m., n=8; lamina-pore complexes: s.e.m., n=7) (^*P<0.01). Adenosine, 200 µM; wortmannin, 1 µM; LY294002, 10 µM.