Fig. 4. PI4K230 is absent in NIH 3T3 and COS-1 cells. Cells were either mock transfected or transfected with cDNA encoding myctagged PI4K230. Proteins from cell lysates from equal amounts of cells were separated on 6% SDS-PAGE for PI4K230 and on 8% SDS-PAGE for PI4Kß and subsequently blotted and incubated with antibodies directed against myc epitope (9E10), PI4K230 and PI4Kß. Anti-PI4K230 was prepared as described in the Materials and Methods. Epitope-tagged PI4K230 was used as a control to demonstrate the expression of PI4K230 in COS-1 cells.