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Fig. 5. The presence of PI4Kß in the nuclear fraction. Proteins from cell equivalents of cell lysate (CL), post nuclear supernatant (PNS) and five times cell equivalents of membrane-depleted nuclei (N) were separated on 8% SDS-PAGE and subsequently western blotted and incubated with antibodies directed against tubulin, PI4Kß and the myc-epitope (9E10). 3T3, proteins from mock-transfected NIH 3T3 cells; CHO, proteins from mock-transfected CHO cells, and CHO + mPI4Kß, CHO cells transfected with epitope-tagged PI4Kß (myc).