Fig. 2. EGF-induced redistribution of Grb2-YFP to the cell periphery in A431 cells. A431 cells were transfected with YFP fused to either wild-type Grb2, the SH2 domain mutant or the SH3 domain mutants of Grb2, as follows. (A,B) Wild-type Grb2-YFP, (C,D) SH2m-YFP, (E,F) NSH3m-YFP, (G,H) CSH3m-YFP and (I,J) NCSH3m-YFP. Images of live A431 cells in each column were taken prior to (A,C,E,G,I) and 1 minute after EGF stimulation (B,D,F,H,J) using confocal microscopy. Bars, 5 µm.