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Fig. 3. Internalization of EGFR-CFP with Grb2-YFP induced by EGF. Individual live COS-7 cells co-expressing wild-type Grb2-YFP and EGFR-CFP were monitored over time by confocal time-lapse imaging. Protein movement of Grb2-YFP (middle) and EGFR-CFP (left) upon EGF stimulation is shown. Arrows point to the characteristic internalized structures (see text).