Fig. 4. Colocalization of Grb2-YFP with clathrin heavy chain, AP-2 and dynamin. COS-7 cells co-expressing EGFR-CFP with wild-type Grb2-YFP were stimulated. Cells were then immunostained with antibodies directed at clathrin heavy chain (CHC), AP-2 or dynamin, followed by a TRITC-conjugated secondary antibody (middle). Arrows point to co-localized structures.