Fig. 6. Internalization of EGFR-CFP is not blocked by the inhibition of clathrin-mediated uptake. COS-7 cells were co-transfected with EGFR-CFP and the C-terminal domain of AP180 and serum starved. In nontransfected cells, transferrin localizes to the perinuclear region. In co-transfected cells, the C-terminal domain of AP180 inhibits clathrin-mediated uptake and transferrin fails to localize (left). When stimulated with EGF, co-transfected cells still take up EGFR-CFP in large structures (right). Images from a time-lapse series are presented. At time 0, EGF was added to a final concentration of 100 ng/ml. Confocal images were captured every 30 seconds. Arrows indicated a region of interest initially on the plasma membrane. The region is internalized and moves to the perinuclear region where it deforms the nuclear envelope.