Fig. 7. Requirement of the intact N-terminal SH3 domain of Grb2 for EGFR internalization. Images of live COS-7 cells co-expressing NSH3m-YFP and EGFR-CFP were collected using confocal microscopy; NSH3m-YFP (left) and EGFR-CFP (middle). Shown at the bottom is a higher magnification view of the boxed region. Arrows point to large spherical structures associated with the plasma membrane (see text). Bar, 5 µm.