Fig. 8. Colocalization of NSH3m-YFP with clathrin heavy chain, AP-2 and dynamin. COS-7 cells co-expressing EGFR-CFP with NSH3m-YFP were stimulated. Cells were then immunostained with antibodies directed at clathrin heavy chain (CHC), AP-2 or dynamin, followed by a TRITC-conjugated secondary antibody. Arrows point to co-localized structures.