Fig. 3. Expression of anti-CD scFvs in mammalian cells. COS cells were transiently transfected with the 372 scFv fragment targeted to the nucleus (372nuc) (A), 372 targeted to the cytoplasm (372cyt) (C) and with the 373nuc (E) and 373cyt (G). Their nuclear localization was determined using anti-myc antibodies. In the corresponding right panel (B,D,F,H) are shown the same cells counterstained with the dye Hoechst 33258, which highlights the A/T-rich repeat sequences present in the prominent heterochromatic regions. Scale bars, 5 µm.