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Fig. 4. In vivo interaction of anti-CD scFvs with endogenous HP1ß protein. (A) COS cells were transiently transfected with DNA encoding scFv fragments and analysed after 48 hours. A western blot analysis was performed with anti-HP1ß MAC 353 of the insoluble pool from COS fibroblasts transfected with anti-ßgal scFv (lane 1). Western blot of soluble proteins from COS cells transfected with anti-ßgal (lane 2), 372nuc (lane 3) and 373nuc (lane 4) immunopurified using anti-myc 9E10 and then probed with anti-HP1ß monoclonal antibody MAC 353 is shown. The arrow points to the HP1ß protein. (B) Western blot analysis of anti-ßgal (lane 1), 372nuc (lane 2) and 373nuc (lane 3) scFv fragments immunopurified from transfected COS cells, viewed with anti-myc IgG. The migration of molecular weight markers (in kDa) is shown on the left and the heavy ({gamma}) and light (k) chains are indicted on the right. The arrow points to the anti-ßgal scFv fragment (lane 1) and the arrowhead points to the anti-CD scFv fragments (lanes 2 and 3).