Fig. 7. Anti-CD scFvs induce p53-independent cell death, which is partly prevented by Z-VAD fmk treatment. (A) Non-transfected cells (nt) and cells transfected with anti-ßgal, 372nuc and 373nuc scFv fragments (as indicated) were counted for their positive staining with Annexin V and Hoechst 33342. The figure shows the average of three different experiments. At least 60 positively transfected cells for each plasmid were counted and checked. (B) Hoechst-33342-positive cells were counted from non-transfected cells incubated with H₂O₂ and cells transfected with different scFvs (as indicated) in the presence or in the absence of the caspase inhibitor Z-VAD fmk.