Fig. 4. NuMA tail IIA stabilises microtubules. Cells expressing GFP-NuMA tail IIA (green) were incubated on ice for 1 hour, fixed and processed for immunofluorescence of tubulin (A, red) or incubated at room temperature and stained for acetylated tubulin (B, red). Chromosomes are stained with DAPI (blue). Note the absence of a microtubule network in the untransfected cell in (A) Bar, 20 µm.