Fig. 6. Full-length NuMA requires tail IIA to associate with tubulin when expressed in the cytoplasm of interphase cells. Cells expressing GFP-NuMA NLS, tail II or tail IIA+NLS (green), fixed and processed for immunofluorescence of tubulin (red). Chromosomes are stained with DAPI (blue). (A) Interphase cells. Note the presence of tubulin aggregates co-localizing with GFP-NuMA NLS (top row), and the absence of tubulin in protein aggregates of the mutants lacking tail II or tail IIA. Bar, 20 µm. (B) Mitotic cells. Bar, 10 µm.