Fig. 1. Typical views of the centrosomes as seen at different time points during the cell cycle when stained by an autoimmune serum (for details, see Ou and Rattner, 2000). (A-C) Diagrammatic representation of the centrosome before duplication (A), the centrosome in the process of duplication (B), and the centrosomes after duplication and separation (C). Arrowheads denote the site from which a new tubular centrosome (daughter centrosome) is formed. The upper panel shows the 3D view of the centrosome. The lower panel illustrates both longitudinal and cross-sectional views of the centrosome tube shown at the upper panel. CV, cross-sectional view; LV, longitudinal view. Note that the longitudinal view represents the middle sections whereas the cross-sectional view represents the top end of the centrosome tube. (D-F) Overlay of whole cell DIC images and the fluorescent centrosomes (red spots) in HeLa cells at the cell cycle stages G1 (D), S (E) and G2 (F). The inserts show high magnification views of centrosomes from the corresponding cells. Note the centrosomes seen at D-F provide the basis for the diagrammatic views of the centrosomes shown in A-C. Bar, 10 µM (for DIC images only).